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Semen Detection Kit
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InSite Semen Detection Kit
The kit is designed to be used by men who suspect their spouse may be engaged in sexual activity outside of their relationship.
It also can be used by professional investigators, and parents concerned about whether their teenage daughters are sexually active.
The InSiteSemen Detection Kit contains 15 AP test strips in a resealable pouch with desiccant, 10 PSA test strips in sealed individual pouches and a 5-mL dropper.
The AP strips can detect semen down to a 1/2000 dilution, while the PSA strips can detect semen to a 1/500,000 dilution.
Sensitivity
Semen may be detected on women's undergarments which has been discharged up to 17 hours after intercourse with the AP test strips, and up to 36 hours after intercourse with the PSA test strips.
The AP test is presumptive, and a positive result should always be followed by a PSA test for confirmation of the presence of semen.
Specificity
The PSA test is 100% specific for semen at a dilution of 1/500,000 or less, this means that the probability of a false positive result is essentially zero within the first 12 hours after intercourse.
In order to be sure that the semen did not come from you, you must not have had intercourse with your spouse for at least three days prior to the test.
Frequently Aksed Questions
Q: How old can the stain be?
A: PSA has been detected in semen stains on garments over 30 years old.
Q: Are all stains semen?
A: NO. Asymptomatic women produce, on the average, about 1.5 g of vaginal fluid per day, which typically leaves a white-to-beige stain.
Semen stains on the other hand, are white and appear mainly just after intercourse. The next day, discharge of residual semen may not be visible at all.
Q: Is this a legitimate forensic test?
A: Both the PSA test and the AP test are used for the forensic detection of semen by professional investigators worldwide.
The PSA test in particular has been validated for use in forensic investigations, and the small amount of PSA present in other bodily fluids has been shown not to interfere with the detection of semen.
Materials supplied in the kit
Materials not supplied but required
Simple procedure
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AP Test:
Place 5-10 drops of water on a suspect area of the garment.
Press an AP strip against it.
A color change to bright purple within the first 60 seconds is a POSITIVE test.
If the test is POSITIVE, proceed with a PSA test to confirm the presence of semen.
If the test is NEGATIVE, but you are suspicious there might be a trace of semen on the garment, do the PSA test anyway.
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PSA Test.
Place 15 mL of water in a coffee cup using the supplied dropper.
Then, manually extract the suspect area (i.e. crotch) of the garment by repeatedly allowing water to soak in, then pressing it out.
Finally, wring out the garment into the cup.
Place a PSA test strip into the cup and wait 10 minutes.
Then, take the test strip out and lay it on a clean dry surface.
Read the test strip after 10 minutes.
A POSITIVE test is indicated by two lines as shown below.
A strongly positive test will be clearly visible within two minutes, while a weakly positive test may take the entire 20 minutes to become evident.
If you are testing absorptive pads (used during a woman's menstrual period), then place 25 mL of water into the coffee cup (for a full pad) or 10 mL for a mini-pad, and repeatedly extract the pad manually.
Then, wring out the pad into the cup and discard it. Do the PSA test as usual.
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